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Ginkgolide B, a major component of Ginkgo biloba , protects against ototoxicity caused by cisplatin in vitro and in vivo by decreasing ROS production through the reduction of NOX2 expression and the enhancement of activity via the Akt/Nrf-2/HO-1 pathway, mitochondrial apoptosis is inhibited (Ma et al

Immunofluorescence tissues were mounted with ProLong Gold (Life Technologies, Cat# P36934), while H&E and immunohistochemistry tissues were mounted with Permount mounting media (Fisher Scientific, Cat# SP15500) and coverslipped for imaging

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Let them air-dry for 5 seconds