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On the other hand, the yeast form of the gdh3 / gdh3 mutant showed a significantly higher intracellular concentration of ATP, NAD + , NADH, NADP + , and NADPH compared to the filamentous form of the wild-type strain in proline medium

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However, due to the high surface energy of single atoms, which leads to agglomeration, most current single-atom loadings remain below 1% to ensure uniform atomic distribution, resulting in limited enzymatic catalytic activity[21, 22]

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